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chemoresistant hose

Silencing of BAG3 promotes the sensitivity of ovarian

16/4/2018· OCCC cell lines were cultured in DMEM/F12 medium (Caisson, Smithfield, UT) and HOSE cell lines were cultured in M199 (Gibco, Grand Island, NY): MCDB105 (Sigma‐Aldrich, St. Louis, MO), (1:1) medium, while HEK293T cells were maintained in Dulbecco''s modified Eagle medium (DMEM) with high glucose and l ‐glutamine (Caisson). All these media were supplemented with 10% fetal bovine serum …

Macrophage Inhibitory Factor-1 (MIF-1) controls the

1/11/2018· Blocking MIF-1 drives cells to dedifferentiate into chemoresistant CD138- cells, and the addition of rMIF-1 drives cells to differentiate into chemosensitive CD138+ cells. These cells can be toggled back and forth depending on MIF-1 activity or levels, offering new insight into the epigenetic control of MM growth. Too little MIF-1 and cells dedifferentiate; too much MIF-1 and cells

(PDF) Human ovarian cancer and cisplatin resistance

Influence of cisplatin on Xiap and Hiap-2 protein expression and apoptosis in hOSE cancer cells. Cisplatin-sensitive A2780s cells and its resistant variant (A2780cp) were cultured in the absence

Pharmaceutical coinations for the treatment of cancer

3 HOSE control cells were generally insensitive to the administration of MPL, however, in two experiments cytotoxicity was recorded at low levels: 5 and 3%, respectively (oxaliplatin and flutamide experiments, see FIGS. 21 and 22, below). 4 Synergy was observed upon HOSE cells only at …

Construction autophagy-related prognostic risk signature

6/3/2021· The brief information of 7 GEO datasets [containing 410 human ovarian surface epithelial (HOSE) Cohn DE, Selvendiran K. Hypoxia-induced exosomes contribute to a more aggressive and chemoresistant ovarian cancer phenotype: a novel mechanism linking STAT3/Rab proteins. Oncogene. 2018;37(28):3806–21. CAS Article Google Scholar 37. Emmings E, Mullany S, Chang Z, Landen CN Jr, …

CHTOP in Chemoresistant Epithelial Ovarian Cancer: A Novel

27/6/2019· CHTOP in Chemoresistant Epithelial Ovarian Cancer: A Novel and Potential Therapeutic Target. OV-90 and HOSE cell lines were maintained in a 1:1 mixture of MCDB 105 and 199 medium (Sigma-Aldrich) supplemented with 15% FBS, 50 U/mL penicillin, and 50 μg/mL streptomycin. All cell lines were maintained in a humidified incubator at 37°C and 5% CO 2. Cell Viability Analysis. 5 × 10 3 cells

Molloy College [email protected] Inhibitions of GSK3β

differentially in chemoresistant and chemosensitive epithelial ovarian cancer patients, as seen in . Figure 1 (Borley & Brown, 2015; Earp & Cunningham, 2015; Lum et al., 2013). DNA methylation is the transfer of a methyl group to the carbon-5 position of cytosine (Lum et al., 2013). This is an example of an epigenetic alteration, as it regulates the temporal and spatial dynamics of a gene and

A2780 Cell Line human 93112519, ovarian carcinoma | Sigma

Cell Line Description. The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (Sigma alogue no. 93112517) and the adriamycin resistant cell line A2780 ADR


The PTFE is induvidual, that is chemoresistant against every acid, alkali, chloride, sulphate and other agents in any concentration from - 70ºC until + 260ºC. Spiral waved PTFE corrugated tubes, especially with alloy steel spiral the big flexibility tube fittings they protection spiral prevents the breaking in case of strong bending. Because of its special characteristics (biggest resistance

"Abstract 2934: GSK3β modulates chemoresistance in

Procedure: Human ovarian surface epithelium (HOSE) 6-3 cell line was utilized, which is characterized as sensitive to carboplatin therapy. The cells were studied in six groups: 1) untreated; 2) treated with Lithium Chloride (LiCl); 3) treated with carboplatin; 4) treated with carboplatin and LiCl; 5) treated with doxorubicin as control; 6) treated with doxorubicin and LiCl as another control

HKU Scholars Hub: Overexpression of αPIX contributes to

Methods: The expression of αPIX in 143 clinical samples of ovarian tumors including inclusion cysts, benign cystadenomas, borderline tumors, carcinomas of different histological subtypes as well as metastatic foci of ovarian cancers was analyzed by immunohistochemistry and real-time PCR. αPIX expression was also assessed in cell-lines of human normal ovarian epithelium (HOSE 11-12 and …

Livin - an overview | ScienceDirect Topics

XIAP content was higher in chemoresistant OVCA cells compared to their sensitive counterparts and CDDP decreased the content in the sensitive but not the resistant cells. The decrease in protein content in the sensitive cells after cisplatin treatment correlated with increased apoptosis [64]. The downregulation of XIAP in the chemoresistant OVCA cells resulted in increased activation of

Stem cell transcription factor NANOG controls cell

3/9/2012· Three immortalized human normal ovarian epithelial cell lines, HOSE 6-3, HOSE 11-12 and HOSE 17-1, and 13 ovarian cancer cell lines, DOV13, ES2, …

XIAP - an overview | ScienceDirect Topics

Expression of exogenous XIAP in cisplatin-sensitive hOSE cells confers resistance to cisplatin-induced apoptosis [31]. These findings further implie XIAP but not c-IAP in conferring sensitivity or resistance to chemotherapeutic drugs in hOSE cells. Cisplatin is a major chemotherapeutic agent for the treatment of hOSE; however, the development of drug resistance by hOSE cells hinders the

Frontiers | CHTOP in Chemoresistant Epithelial Ovarian

Objective: Chemoresistance is a major challenge in epithelial ovarian cancer (EOC) treatment. Chromatin target of protein arginine methyltransferase (CHTOP) was identified as a potential biomarker in chemoresistant EOC cell lines using label-free LC-MS/MS quantitative proteomics. Thus, the aim of this study is to investigate the role of CHTOP in chemoresistant EOC and the underlying mechanism

Bitter Melon (Momordica charantia) Extract Inhibits

19/10/2015· Ovarian cancer cell lines, human immortalized epithelial ovarian cells (HOSEs), and nude mice were used to evaluate the cell cytotoxicity, cisplatin resistance, and tumor inhibitory effect of BME. The molecular mechanism of BME was examined by Western blotting. Results. Cotreatment with BME and cisplatin markedly attenuated tumor growth in vitro and in vivo in a mouse xenograft model, …

Involvement of classic and alternative non-homologous end

3/11/2021· Chromosomal transloions are the main etiological factor of hematologic malignancies. These transloions are generally the consequence of aberrant DNA double-strand break (DSB) repair. DSBs arise either exogenously or endogenously in cells and are repaired by major pathways, including non-homologous end-joining (NHEJ), homologous recoination (HR), and other minor pathways …

Down-Regulation of X-linked Inhibitor of Apoptosis Protein

15/10/2000· Xiap down-regulation not only induces apoptosis but also sensitizes chemoresistant wild type p53 hOSE cancer cells to the proapoptotic action of cisplatin. In p53 mutant cancer cells, coexpression of Xiap antisense and wild type p53 sense is more effective than wild type p53 restoration alone. These findings provide a new concept for the development of novel therapeutic approaches in …

Ginger inhibits cell growth and modulates angiogenic

20/12/2007· Ginger treatment resulted in similar effects in all cell lines tested, including the chemoresistant cell lines SKOV3 and ES-2 Untransformed human ovarian surface epithelial cells (HOSE) were minimally affected by ginger extract exposure at days 1 and 3, and showed some inhibition in growth by day 5 (Figure (Figure1D, 1D, p > .05 for days 1 and 3, p < .05 for day 5). To confirm that …

Attributes of Oct4 in stem cell biology: perspectives on

21/11/2012· hals M, Kassaara A, De Vos J, Hose D, Moreaux J, Klein B: Eryonic stem cell markers expression in cancers. Biochem Biophys Res Commun 2009, 383: 157–162. 10.1016/j.bbrc.2009.02.156. CAS PubMed Article Google Scholar 40. Viswanathan SR, Powers JT, Einhorn W, Hoshida Y, Ng TL, Toffanin S, et al.: Lin28 promotes transformation and is associated with …

Down-regulation of X-linked Inhibitor of Apoptosis Protein

In this context, we have recently observed that down-regulation of Xiap by adenoviral antisense expression not only induced apoptosis in chemoresistant hOSE cancer cells (C13) in vitro, but also

New chondrosarcoma cell lines and mouse models to study

19/8/2013· Saini V, Hose CD, Monks A et al. Identifiion of CBX3 and ABCA5 as putative biomarkers for tumor stem cells in osteosarcoma. Identifiion of CBX3 and ABCA5 as putative biomarkers for tumor

S‐(4‐Methoxyphenyl)‐4‐methoxybenzenesulfonothioate as a

13/12/2019· A great challenge for antitumor therapies is the genetic heterogeneity of advanced cancer because many cancer cells can develop resistance to chemotherapeutic drugs. 6 Metastatic renal cell carcinoma is especially chemoresistant. 7 The resistance shown by these cells may be related to their high concentration of intracellular glutathione (GSH). 8 Renal carcinoma cells are known for producing …

Down-Regulation of X-linked Inhibitor of Apoptosis Protein

antisense Xiap-induced apoptosis in the chemoresistant hOSE cancer cells was associated with caspase-3-mediated MDM2 cleavage and increased p53 content. These findings suggested that Xiap is not only regulating down-stream events of caspase-3, but also is triggering p53-dependent pathway when down-regulated by antisense and/or cisplatin treatment. Received 2/18/00; accepted 8/16/00. The costs

My Sister''s Keeper - Jodi Picoult ~ Chương 2 # mobile

I am not certain, yet, that I will take a hose up. This furnace was designed to consume remains at 1,600 degrees Fahrenheit. There is fire above and below the body. “Well?” the dean says. “Aren''t you going to do something?” It is the biggest mistake rookies make: the assumption that fighting a fire means rushing in with a stream of water. Sometimes, that makes it worse. In this case

iASPP and Chemoresistance in Ovarian Cancers: Effects on

A, mRNA and B, protein expression pattern of iASPP in HOSE cell lines and ovarian cancer cell lines. C, higher iASPP expression was found in the chemoresistant patients than in the chemosensitive patients. D, iASPP protein expression in 4 pairs of chemosensitive and resistant ovarian cancer cell lines were compared with higher expression shown in chemoresistant cell lines (PEA2, PEO23). All